翻译一：

Globinexpressing E. coli cultures were grown in shake flasks at 37°C and at 200 rpm for 4 h followed by a period of 10 h at 100 rpm, on Luria-Bertani (LB) medium supplemented with 100 mg L-1 ampicillin. Cells from total of 3 L of culture were harvested, resuspended in 100 mM potassium phosphate buffer, pH 7.0, containing 1?M phenylmethanesulfonyl fluoride (PMSF) to reach an OD600 ) 600, and lysed in a French press (SLM-Aminco)

翻译：Globinexpressing大肠杆菌文化是生长在摇瓶在37℃和200由后10小时内为100转4小时，在卢里亚-博尔塔尼（磅）中补充100毫克L - 1的氨苄青霉素转速。从3文化L总收获细胞，悬浮在100毫米的磷酸钾缓冲液，pH值7.0含1?M phenylmethanesulfonyl氟（PMSF）达成一项OD600）600，并在法国报刊裂解（苏丹解放运动Aminco）

翻译二：

All protein purification steps were performed at 4 °C. Lysates were clarified by centrifugation

and filtration (0.45?m). His-tagged Hb and flavoHb proteins were purified on an affinity column, Sephadex HisTrap chelating column (Amersham Pharmacia Biotech),and eluted stepwise in imidazole buffer (40 and 300 mM imidazole in 100 mM potassium phosphate buffer, pH 7.0) followed by a desalting column (Sephadex G25). Sample purity was estimated to be >95% for bacterial Hbs and

>90% for flavoHbs from SDS-12% polyacrylamide gels stained in Coomassie Brillant Blue

翻译：所有蛋白质纯化步骤进行，4℃裂解澄清了离心

和过滤（0.45?米）。他的标记血红蛋白和flavoHb蛋白质纯化上的亲和柱，葡聚糖HisTrap螯合柱（分离液），在100毫米钾磷酸盐缓冲液，pH值7.0咪唑缓冲液（40和300毫米咪唑洗脱逐步）由脱盐后列（葡聚糖达东路）。样品纯度，估计是“95％的细菌血红蛋白

“90％的从SDS flavoHbs - 12％聚丙烯酰胺凝胶染色考马斯亮蓝

翻译三：

FAD content was assayed by releasing FAD from flavoHb by boiling the purified protein sample for 3 min and determining the fluorescence at 520 nm with excitation at 460 nm, by comparison with pure FAD as a standard.

翻译：基金会含量测定基金会由flavoHb，煮沸3分钟纯化的蛋白质样品和520纳米的激发荧光的决定释放460纳米，与作为标准的比较纯粹的基金会。

The most enchanting of mystical creature ?

可翻译为:

最迷人的神秘生物。

enchanting作为形容词，可表示迷人的意思。

enchanting 是动词enchant的现在分词形式。

enchant 作为动词，可表示施魔法于，吸引等意思。

例句有:

You'll have to enchant the ball with a magic spell before you can use it.

在你使用魔法球之前，你必须用口诀对其施展魔法。

例句配图

mystical 作为形容词，可表示神秘的，奥秘的等意思。

例句有:

Watching the sun rise over the mountain was an almost mystical experience .

看着太阳爬上山岗，这几乎是一种难以言传的体验。

例句配图

creature作为名词，可表示生物；动物；人；创造物等意思。

例句有:

This creatute comes from outer space .

这是一只外星生物。

例句配图